A monolithic HPLC column, also known as a monolithic column, is a high-performance liquid chromatography column (HPLC). The monolithic column's interior structure is designed in such a way that several channels form within it. The material that divides the channels inside the column can be permeable and functionalized. Most HPLC designs, on the other hand, use particle packed columns, which include microscopic beads of an inert material, often modified silica, inside the column. Monolithic columns are divided into two types: silica-based monoliths and polymer-based monoliths. Silica-based monoliths excel in separating smaller molecules, while polymer-based monoliths excel at separating large protein molecules.<br /><br />MSc Salam Mohammed Naser