Training Workshop on the Use of the Light Microscope and Sample Examination in the Department of Medical Laboratory Sciences

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The Department of Medical Laboratory Sciences organized a distinguished training workshop on the use of the light microscope and sample examination, attended by six participants: Ms. Duaa Fadhel, Ms. Baneen Turki, and Ms. Zainab Mahmoud. The workshop aimed to enhance students’ practical skills in handling electron microscopes and examining samples with accuracy and professionalism. The workshop included a detailed explanation of the types of electron microscopes, introducing the Transmission Electron Microscope (TEM), which allows examination of the internal structure of samples using ultra-thin sections, and the Scanning Electron Microscope (SEM), which enables the study of surface and three-dimensional external structures without the need for very thin samples. The workshop also focused on sample preparation steps, considered among the most important stages for obtaining accurate results, including: • Fixation: Using Glutaraldehyde followed by Osmium tetroxide for biological samples to preserve cellular structure and prevent degradation. • Dehydration: Through a graded series of alcohols from 30% to 100%. • Coating (SEM only): Covering the sample with a layer of gold or platinum to make it conductive. • Sectioning (TEM only): Using an ultramicrotome to obtain ultra-thin sections. Additionally, the workshop covered electron microscope examination procedures, starting with placing the sample inside a vacuum chamber, activating the electron source, adjusting magnification, focus, and beam intensity, and finally capturing images and analyzing them digitally. Safety precautions were also emphasized, due to the use of toxic chemicals such as Osmium, avoiding moisture inside the device, and refraining from touching samples directly with hands. The workshop concluded with a review of the applications of electron microscopes, including studying microorganisms, medical examination of tissues and cells, materials research (metals, polymers, nanomaterials), as well as forensic and research applications. The participants expressed their keen interest in maximizing the benefits of the workshop to enhance their practical and theoretical knowledge in the field of medical laboratory sciences, praising the importance of such workshops in developing students’ practical skills and linking them to theoretical studies. Al-Mustaqbal University is the first university in Iraq Department of Medical Laboratory Techniques – First in the Iraqi National Ranking.