Introduction:<br />At a crime scene, biological fluids may be present that are not visually identifiable. Digestive enzyme analysis helps accurately identify the type of fluid.<br /><br />Context:<br />Each body fluid contains distinct enzymes:<br /><br />Saliva contains amylase<br /><br />Urine contains urease<br /><br />Semen contains acid phosphatase<br /><br />Using special chemical reagents, the source of an unknown stain can be identified.<br /><br />Forensic Application:<br /><br />Determining whether a stain is saliva, blood, or semen<br /><br />Supporting evidence in rape or violence cases<br /><br />Confirming a person's presence at a particular location based on the type of fluid<br /><br />Conclusion:<br />Enzyme analysis provides high accuracy in identifying biological fluids, enhancing the reliability of biological evidence in investigations.<br /><br />Al-Mustaqbal University – The First University in Iraq